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KMID : 0381920080380040285
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Korean Journal of Microscopy
2008 Volume.38 No. 4 p.285 ~ p.291
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Objective Aperture Effects for the Quantitative Analysis in Electron Tomography
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Kim Jin-Gyu
Lee Sang-Hee
Kweon Hee-Seok
Jeong Jong-Man
Jeong Won-Gu
Lee Su-Jeong
Jou Hyeong-Tae
Kim Youn-Joong
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Abstract
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We have evaluated the effects of experimental factors on transmitted electron beam intensities for quantitative analysis
in electron tomography. For the correct application of Beer’s law in electron tomography, the transmitted beam intensity should reflect the net effect of mass properties on beam path. So, the any other effects of the objective aperture and the specimen holder on beam path should be removed. The cut-off effects of objective aperture were examined using Quantifoil holey carbon film and a transmission electron microscope operated at 120 kV. The transmitted beam intensities with 30 μm objective aperture dropped about 16.7% compared to electron beam intensities without the objective aperture. Also, the additional losses of about 14.2% at high tilt angles were occurred by cut-off effects of the objective apertures. For the precise quantitative analysis in electron tomography, the effect of the objective aperture on transmitted electron beam intensities should be considered. It is desirable that 2-D tilt series images are obtained without the objective aperture for correct application of Bee’s law.
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KEYWORD
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Beer’s law, Cut-off effect, Electron tomography, Objective aperture
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